When we compared the corresponding amino acid sequences of the putative cadF (-like) ORF from the 17 C. lari and some C. jejuni isolates with this consensus motif, the motif was completely conserved amongst https://www.selleckchem.com/products/px-478-2hcl.html the cadF (-like) ORFs from the isolates (data not shown). As shown in Table 2,
the CMW of the putative cadF (-like) ORF was estimated to be 36,578 to 36,869 Da for the 16 C. lari isolates and C. lari RM2100 reference strain (data not shown). In addition, the value was also estimated to be approximately 36 kDa for the two C. jejuni reference strains (Table 2). These estimated CMW values are in agreement with the previous description of the immunodetection of the CadF protein
from five C. jejuni and C. coli Captisol solubility dmso isolates . When the nucleotide and deduced amino acid sequence alignment analyses were carried out for the putative cadF (-like) ORF, apparent size selleck products differences occurred amongst the four thermophilic Campylobacter species, as described above. Regarding the putative ORFs for cadF (-like) gene between C. lari and C. jejuni organisms, nine amino acid residues are shorter in C. jejuni strains than in C. lari isolates. Recently, Krause-Gruszczynska et al. (2007) described that the CadF protein from C. coli strains was 13 amino acid larger than those from C. jejuni strains, based on the deduced amino acid sequence alignment analysis . This is consistent with our present results (Table 2). They also indicated that C. coli strains carried a stretch of 13 amino acid in the middle region of the protein . In addition, in the present study, the deduced CadF (-like) protein was shown to be 328 amino acid from all 17 C. lari isolates
and were nine amino acid larger than CadF from two C. jejuni strains (319 amino acid) (Table 2). Then, we carried out deduced amino acid sequence alignment analysis to elucidate the differences in CadF (-like) protein between C. lari and C. jejuni organisms. As shown in Figure 3, the Rebamipide C. coli RM2228 strain carried a stretch of 12 amino acid (VVTPAPAPVVSQ) from amino acid positions 190 to 201 as well as a Q at amino acid position 180 (Figure 3). In relation to the nine larger amino acid for C. lari isolates than C. jejuni strains, interestingly, four amino acid sequences (THTD) from amino acid positions 80 to 83 and five [A(T for UPTC99) KQID] from 193 to 197 were identified, as shown in Figure 3. Regarding the CadF in Campylobacter, the cadF virulence gene, encoding 37 kDa outer membrane protein that promotes the binding of the pathogens to intestinal epithelial cells, was identified and cloned [22, 25]. In relation to identification of the binding domain within C. jejuni CadF, Konkel et al.