This finding agrees with other study where two lactobacilli were able to increase the cell surface expression of TLR5 in HT29 cells to respond to S. Typhimurium [10]. In our model, this receptor could be also implicated in the protective effect of L. casei CRL 431 against see more S. Typhimurium infection. Finally, in our study, it was observed that L. casei CRL 431 oral administration increased TLR9 expression in healthy mice (Figure 3D). Seven days post infection, the increase of TLR9 (+) cells was observed in both groups of mice given probiotic bacteria (Lc-S and Lc-S-Lc), but

not in the infection control (S group), comparing with the untreated control group (C). This finding agrees with several works which affirm that CpG-TLR9 interaction can improve the resistance of normal adult mice to a variety of bacterial, viral and parasitic pathogens [36–38], including increased resistance to oral challenge with S. Typhimurium. TLR9 signalling is also required to mediate an anti-inflammatory

effect induced by probiotics, in a mouse colitis model [39]. Conclusions The results of the present work demonstrated the importance of L. casei CRL 431 continuous administration, before and after S. Typhimurium infection, to maintain the mechanisms of protection against this pathogen. L. casei CRL 431 administration before infection maintained the innate immune system in alert state, through modulated Enzalutamide expression of TLRs and cytokine signals in the effector and inductor site of the

gut immune system, which could be related with the protection against S. Typhimurium observed in a previous report. The results from the present work show that once established the disease, the continuous diglyceride L. casei CRL 431 administration protected the host mainly modulating the inflammatory response against the enteropathogen in both effector and inductor sites of the gut. This preliminary study shows some of the immune mechanisms implicated in the protective effect of L. casei CRL 431 againts S. Typhimurium infection. More studies should be performed to validate the use of this probiotic Anlotinib molecular weight strain in the prevention and as a complement to treatments in the defense against salmonellosis. The cellular populations involved in the cytokine production and how TLRs activate the different signals and the transcriptional factors for cytokine production are currently under study. Methods Animals and experimental groups Five-week-old BALB/c mice weighting 22-26 g were obtained from the closed random bred colony maintained at CERELA (Centro de Referencia para Lactobacilos, San Miguel de Tucumán, Argentina). The assays were performed using 3 experimental groups to assess the effect of the preventive or continuous probiotic administration against S. Typhimurium infection comparing with the infection control group (S).