The purpose of this study was to detect HPV-DNA in intraosseous ameloblastomas.
Methods. Eighteen cases of intraosseous ameloblastomas click here of different histological variants were selected. Immunohistochemistry, CISH, nested-PCR, and INNOLiPA HPV Genotyping v2 were used.
Results. The predominant age group was between the third and fourth decades of life. Males were more affected with 61% and females represented 39%. Of the 18 cases, 7 were solid multicystic tumors and 11 were unicystic. Of the histological variants, the plexiform represented 3 (17%) of the 18, 2 (11%) were follicular, 2 (11%) were acanthomatous, and 1 (6%) was desmoplastic. All cases were HPV negative by immunohistochemistry and CISH. HPV-DNA
was detected in 6 (33%) of the cases by nested-PCR. HPV 6 was the most frequent β-Nicotinamide clinical trial genotype in 4 (66%) of the 6. Two cases presented a mixture of HPV 16, 33, and HPV 6, 42 respectively. Four of the unicystic ameloblastomas were HPV positive; of these, all presented koilocytic
changes and were associated with dentigerous cysts, whereas only 2 positive cases corresponded to solid ameloblastomas. None of the positive cases were related to recurrence rate.
Conclusions. We may conclude that HPV low and high risk was detected in our sample of intraosseous ameloblastomas. HPV positivity was observed more in the unicystic cases than solid types. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010;110:e20-e24)”
“Aicardi-Goutieres syndrome is an early-onset encephalopathy with a presumed immune pathogenesis caused by inherited
defects in nucleic acid metabolism. The clinical picture resembles a congenital viral infection despite negative investigations Selleckchem GSK690693 for common viruses. In addition to leukoencephalopathy with calcifications of basal ganglia, patients show increased levels of the antiviral cytokine interferon-alpha in cerebrospinal fluid. We report on a 12-year-old boy with Aicardi-Goutieres syndrome and systemic lupus erythematosus (SLE) due to mutations in the SAMHD1 (sterile alpha motif domain and HD domain-containing protein 1) gene, illustrating an emerging pattern of the natural history of Aicardi-Goutieres syndrome characterized by neurological disease followed by symptoms of systemic autoimmunity. Thus, Aicardi-Goutieres syndrome constitutes a model disease for systemic autoimmunity triggered by the activation of the innate immune system. Recognition of the etiologic link between Aicardi-Goutieres syndrome and systemic lupus erythematosus has direct implications on therapeutic management and suggests that early immune modulatory intervention can improve neurological outcome.”
“A particle-in-cell/Monte Carlo model is developed to study and analyze the electrical characteristics of the nonequilibrium plasma created by radio frequency (RF) discharge in Ar/O-2 mixtures in the presence of crossed electric and magnetic fields. The method of collision treatment is based on an optimized estimation of the free time flight.