Roseobacter, Rhodobacteraceae) were similar to those found in previous aquatic biofilm studies using glass slides (Dang & Lovell, 2000; Jones et al., 2007). In summary, this study suggests that when biofilms are subjected to long-term deployment (weeks to months), as presented here, simple glass slides enable the formation of bacterial biofilm communities that are highly similar to other ‘natural’ substrates such as coral skeletons or reef sediment grains.
Additional advantages for the use of glass slides include a standardized size, low cost, ease of handling and the formation of relatively reproducible selleck screening library bacterial community structures among replicates. This study therefore also provides further evidence that monitoring bacterial communities associated with coastal biofilms may find application as a bio-monitoring tool for environmental management for examining local and regional changes in water quality in the long-term. Future work should include more in-depth studies of the bacterial communities grown in different water
qualities over replicate seasons. We thank C. Humphrey, C. Reymond, F. Patel and J. van Dam for assistance this website in the field and the crew of the R.V. Cape Ferguson for the assistance during fieldwork. The water quality data were collected as part of the Reef Plan Marine Monitoring Program, which is supported by the Great Barrier Reef Marine Park Authority (GBRMPA) through funding from the Australian Government’s Caring for our Country and by the Australian Institute of Marine Science (AIMS). We are grateful to I. Zagorskis for summarizing the water quality data and K. Wasmund for his critical and helpful comments on the manuscript. This project (project 3.7.1) was funded by
Protein kinase N1 the Australian Government Marine and Tropical Sciences Research Facility (MTSRF). “
“Pigs from a variety of sources were surveyed for oro-gastrointestinal (oro-GIT) carriage of Candida albicans. Candida albicans-positive animals were readily located, but we also identified C. albicans-free pigs. We hypothesized that pigs could be stably colonized with a C. albicans strain of choice, simply by feeding yeast cells. Piglets were farrowed routinely and remained with the sow for 4 days to acquire a normal microbiota. Piglets were then placed in an artificial rearing environment and fed sow milk replacer. Piglets were inoculated orally with one of three different C. albicans strains. Piglets were weighed daily, and culture swabs were collected to detect C. albicans orally, rectally and in the piglet’s environment. Stable C. albicans colonization over the course of the study did not affect piglet growth. Necropsy revealed mucosally associated C. albicans throughout the oro-GIT with the highest abundance in the esophagus. Uninoculated control piglets remained C. albicans-negative. These data establish the piglet as a model to study C. albicans colonization of the human oro-GIT.