L Solutions were used to spontaneous excitatory and inhibitory postsynaptic beaches watching me, respectively. The patch pipettes had a resistance of 10 20 MX. The sEPSCs were at a holding potential of -70 mV, where no recorded sIPSCs observed because the reversal potential was approx for sIPSCs Hr 70 mV. On the other side were measured at a sIPSCs VH of 0 mV, where sEPSCs invisible because of the observed reversal potential for sEPSCs too close to 0 mV. Cs and tea were added to inhibit K channel located in recorded SG neurons, and therefore easily pass on VH 0 mV from resting membrane potential. The signals Rocuronium Zemuron were acquired using an Axopatch 200B amplifier Stronger. Current in lock mode voltage can be obtained low-pass filtered at least 3 kHz, 500 kHz and digitized using an A / D converter. The data were stored and analyzed with a PC using pCLAMP 9.2 software. sEPSC and sIPSC events were recorded and analyzed with Mini Analysis Program version. 6.0.3 The criteria for the recognition of the events included a threshold of 5 pA case, its fast rise time and a decay curve, the lengths an exponential decay Hert. The drugs were perfused an L Solution, the drug known concentration without one Change in infusion rate and temperature is applied. The L Ml of solution in the recording with a volume of 0.5 was YOUR BIDDING replaced within 15 s. The drugs used were bicuculline methiodide, strychnine nitrate and flumazenil. JM was in 1232, were kindly gifted by Maruishi Pharmaceutical Co. These drugs first used in distilled water at 1,000 times the concentration of, gel St and subsequently End at 20 ° C. The stored Stamml Was sung to the desired concentration in cancer -L solution diluted immediately before use.
The numerical data are expressed as mean ± SEM. Statistical significance was determined as 0.05 percent in pairs or unpaired Student’s t-test. In all cases F, N is the number of neurons examined. Third Whole-cell patch-clamp recordings were results from a total of 26 SG neurons. Stable recordings nnten k Of slices can be obtained in vitro, maintained for 12 h, and recordings were made from Aldosterone SG neurons for up to 1 h. All neurons had resting membrane potential tested SG of less than 60 mV when measured in current clamp mode. 3.1. JM 1232 Effect of spontaneous excitatory transmission in SG neurons superfused JM 1232 for 2 min had no effect on the Haltestr Me and spontaneous excitatory transmission, as seen inFig. 1B. Fig. 1C, the cumulative distributions of the distance between the event and the amplitude of the sEPSC shows in contr And the size Enordnung of 3 min after the start of superfusion JM 1232nd These distributions were significantly affected in 1232 are from JM was best in six other neurons CONFIRMS. Fig. 1D shows the mean values of frequency and amplitude sEPSC about 3 min after the start of JM 1232 hypothermia, with respect to a controlled The. % These values were not significantly different from 100%. The decay phase of the sEPSC was also JM 1232 Invariant changed, half-life was approximately 3 min after the start of the 1232 JM hypothermia 99 2% of contr Am. 3.2. JM 1232 effect of spontaneous inhibitory transmission in SG neurons There are two types of sIPSCs either GABA or glycine receptors, which are of different lengths, in SG neurons mediated.