T with picrotoxin noncompetitive GABAA receptor antagonist and flumazenil, a benzodiazepine antagonist and flunitrazepam binding to benzodiazepine binding site on the GABA receptor A. millefolium Noting that apigenin is an important component of extracts of Achillea and that previous studies have angstl send effect of this compound as proposed, the effect of apigenin was also PARP Inhibition studied in a dose range similar to what would fall, Achillea millefolium extract in the erh Hten maze and marble burying test can be found. Second Materials and methods 2.1. Achillea millefolium plant material used in our experiments were completed in July 2007 from the Botanical Garden Campus of the Universidade Paranaense Umuarama, Brazil, collected on a Seeh Height of 430 m above sea level. The plant was identified by Dr. Maritza Romagnolo Barion. The samples were deposited at the herbarium of the University of t. 2.2. Production of alcoholic extract of aerial parts were air dried in an oven Achillea at 40 for 4 days and then the dried plant material was cut and sprayed. The dry, powdered plant material was left immersed for 7 days with 90% ethanol as L Solvents. The L Solvent is then removed using a vacuum rotary evaporator under reduced pressure and lyophilized to an extract of 17.4% in dry matter. The alcoholic extract was poured into a 5% w Ssrigen L Solution of Tween 80 gel St and administered in doses of 30 to 600 mg / kg. 2.3. Animals Animals were adult albino Swiss-M Nnchen mice from our breeding M. They were in groups in polypropylene-K Sional with Hobelsp NEN as bedding, housed under a contr On 12 H/12 H-controlled light / dark cycle and temperature EEA. The animals had free access to water and food, with the exception of 1 h before and may need during the experiments. The animals were not specifically addressed prior to the experiments, with the exception handling in animal care and need the drug.
2.4. Medications and treatments, the water-alcohol extract of yarrow, Achillea millefolium diazepam and vehicle were administered by gavage 1 h before the test. Flumazenil and picrotoxin were administered intraperitoneally 30 min before administration of the hydroalcoholic extract of Yarrow. All treatments were in a constant volume of K Body weight 10 ml / kg. Yarrow was dose mice on a vorl Ufigen administered pharmacological screening protocol with different doses of the extract M. used at all doses, no signs of acute toxicity t observed. 0.1% 1.1%: The doses of apigenin were on the content of apigenin calculated in research chemicals library Achillea extract. 2.5. Behavioral tests in acute toxicity t experiments Each animal was tested only in a behavioral test, w min While in the chronic experiment, the same animal in the open field 5 tested before the test was increased in the Hten maze. The Mice were HNT observation room 1 h before the test weight. The procedures in this study were used were in accordance with the guidelines for animal experiments Col care from Brasileiro de Gio Experimentac Animal and the United States National Institutes of Health Guide for the Care and Use of Laboratory Animals. The experimental protocol was approved by the Ethics Committee of the University.