Other moving parts of the process, the cell maturation, or F Promotion of hair cell death or precursor Bank cells prevents hair cells. W While our selection and dose-response experiments, we studied the regeneration of hair cells at 48 hours after treatment. To define more precisely when regeneration inhibitors right, we evaluated the effects of inhibitors of cell on the hair replacement over a period of 72 h period. As shown in Figure 7, both 5 M and 10 M topotecan flubendazole, potent inhibitors of Histamine Receptor regeneration w During the 72 h tested, little or no GFP hair cells in neuromasts have been observed at any time during the course of the rated. In the absence of treatment with neomycin, flubendazole showed low cellular Toxicity re t hair before 72 hours, after which there was a slight but significant decrease in the number of hair cells. Topotecan alone started significantly kill the hair cells after 48 h of treatment with the death of hair cells complete 72 hours. The inhibitory effect of topotecan are noticeable within 24 h inhibit regeneration after treatment with neomycin, w Toxicity during the entire t of the drug had no significant effect up to 48 h, suggesting that this drug does, in fact, the machines are involved in regeneration. Thus seems flubendazole act primarily on the regeneration w topotecan during the regeneration, by two modes: one that occurs tt in the regeneration and perhaps sp block ter influenced the differentiation of hair cells or hair cells mature. Were produced in the samples with 5 M fulvestrant, regeneration of hair cells occur, but there is a slight decrease or delays the delay time in the number of hair cells after 48 h and 72 h. Analysis of these data by two way ANOVA revealed significant main effects of group and time, and a significant interaction.
Pairwise comparisons revealed not a very significant decrease in the production of hair cells by fulvestrant at 48 h and 72 h of recovery after treatment with neomycin and a marginally significant decrease in the number of hair cells by fulvestrant treatment for 72 h in the groups treated with neomycin before. Effect of inhibitors on proliferation, we subsequently determined End when flubendazole, topotecan and fulvestrant adversely Mighty proliferation may need during the regeneration. After exposure to neomycin, fish were l T for 24 h in the EC with 5 M BrdU and the inhibitor to recover. Previous studies show that the majority of cell proliferation support tt after exposure to neomycin and the majority of new hair cells of mitotic events occur within 24 h of exposure begins to neomycin. After the 24 hours of incubation, the number of cells, we GFP, GFP / GFP cells and BrdU / BrdU cells in neuromasts evaluated seven of the 10 fish per group. GFP / BrdU cells were suspected because of dividing cells to be support. Counts of hair cells in the absence of neomycin-induced regeneration is not affected by 5 M flubendazole or fulvestrant, but decreased significantly with 10 M topotecan. In particular, some GFP cells BrdU in and around the neuromasts of simulacra controlled installed Locked GE, indicating that cell division happens next in the absence of bulk products to. However, there are six times decrease in the number of GFP / BrdU cells with either flubendazole or topotecan shows that these drugs fa To inhibit dramatically the division at least a subset of the supporting cells, the absence of damages caused to hair cells.