The effects of those mutations were determined with three differe

The effects of those mutations were determined with three different in vitro assays of pseudouridylation and several tRNA substrates. Surprisingly, it was Selleck Wnt inhibitor found that each of these components of the hPus1p

active site could tolerate certain amino acid substitutions and in fact most mutants exhibited some activity. The most active mutants retained near wild-type activity at positions 27 or 28 in the substrate tRNA, but activity was greatly reduced or absent at other positions in tRNA readily modified by wild-type hPus1p.”
“Only few selected cancer cells drive tumor progression and are responsible for therapy resistance. Their specific genomic characteristics, however, are largely unknown because high-resolution genome analysis is currently limited to DNA pooled from many cells. Here, we describe a protocol

for array comparative genomic hybridization (array CGH), which enables the detection of DNA copy number changes in single cells. Combining a PCR-based whole genome amplification method with arrays of highly purified BAC clones we could accurately determine known chromosomal changes such as trisomy 21 in single leukocytes as well as complex genomic imbalances of single Ulixertinib concentration cell line cells. In single T47D cells aberrant regions as small as 12 Mb were identified in most cases when compared to non-amplified DNA from 10(6) cells. Most importantly, in single micrometastatic

selleck chemicals cancer cells isolated from bone marrow of breast cancer patients, we retrieved and confirmed amplifications as small as 4.4 and 5 Mb. Thus, high-resolution genome analysis of single metastatic precursor cells is now possible and may be used for the identification of novel therapy target genes.”
“The bright blue emission at 420 nm, originating from the charge transfer transition of the self-activated SnO6 octahedron, was observed in Ca3SnSi2O6 host matrix. Bi3+, Dy3+ and Eu3+ exhibited their characteristic emission under ultraviolet excitation when these activators were doped into Ca3SnSi2O6, respectively. The emission intensity located at the blue-yellow and red region of Dy3+ and Eu3+ could be further improved when Bi3+ was co-doped in Dy3+, Eu3+ doped Ca3SnSi2O9. Furthermore, a tunable color emission, especially the white light, was realized in Ca3SnSi2O6: Bi3+, Dy3+, Eu3+ phosphors by adjusting the relative concentration of the activators. It demonstrates that Bi3+, Dy3+, Eu3+ codoped Ca3SnSi2O6 could potentially be used as a tricolor phosphor for the fabrication of phosphor-converted light emitting diodes (pc-LED). (C) 2013 Elsevier B.V. All rights reserved.

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