The dN/dS ratios were computed for all pairs of alleles differing
more than 1%, in order to give an estimate of the allelic divergence, excluding the anomalous dN/dS ratios of those pairs being very similar. The average of the obtained dN/dS values and respective standard deviations are summarized in Table 4. The dN/dS values for the three genes in the MRSA, MSSA and MRSA/MSSA partitions were well below 1 (between 0.08 and 0.25 with standard deviations between 0.05 and 0.1), which suggests a negative or purifying selection acting on the bla locus. In agreement with the average number of SNP learn more per allele, the dN/dS ratios were significantly higher for the blaR1 gene (0.24 – 0.25) and lower for
mecI (0.08 – 0.11). Discussion The rationale for this study comes from several observations strongly suggesting a role of bla genes in the acquisition, stabilization and regulation of mecA gene, the central element {Selleck Anti-diabetic Compound Library|Selleck Antidiabetic Compound Library|Selleck Anti-diabetic Compound Library|Selleck Antidiabetic Compound Library|Selleckchem Anti-diabetic Compound Library|Selleckchem Antidiabetic Compound Library|Selleckchem Anti-diabetic Compound Library|Selleckchem Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|buy Anti-diabetic Compound Library|Anti-diabetic Compound Library ic50|Anti-diabetic Compound Library price|Anti-diabetic Compound Library cost|Anti-diabetic Compound Library solubility dmso|Anti-diabetic Compound Library purchase|Anti-diabetic Compound Library manufacturer|Anti-diabetic Compound Library research buy|Anti-diabetic Compound Library order|Anti-diabetic Compound Library mouse|Anti-diabetic Compound Library chemical structure|Anti-diabetic Compound Library mw|Anti-diabetic Compound Library molecular weight|Anti-diabetic Compound Library datasheet|Anti-diabetic Compound Library supplier|Anti-diabetic Compound Library in vitro|Anti-diabetic Compound Library cell line|Anti-diabetic Compound Library concentration|Anti-diabetic Compound Library nmr|Anti-diabetic Compound Library in vivo|Anti-diabetic Compound Library clinical trial|Anti-diabetic Compound Library cell assay|Anti-diabetic Compound Library screening|Anti-diabetic Compound Library high throughput|buy Antidiabetic Compound Library|Antidiabetic Compound Library ic50|Antidiabetic Compound Library price|Antidiabetic Compound Library cost|Antidiabetic Compound Library solubility dmso|Antidiabetic Compound Library purchase|Antidiabetic Compound Library manufacturer|Antidiabetic Compound Library research buy|Antidiabetic Compound Library order|Antidiabetic Compound Library chemical structure|Antidiabetic Compound Library datasheet|Antidiabetic Compound Library supplier|Antidiabetic Compound Library in vitro|Antidiabetic Compound Library cell line|Antidiabetic Compound Library concentration|Antidiabetic Compound Library clinical trial|Antidiabetic Compound Library cell assay|Antidiabetic Compound Library screening|Antidiabetic Compound Library high throughput|Anti-diabetic Compound high throughput screening| of “”broad-spectrum”" β-lactam resistance characteristic of MRSA strains. The purpose of this study was to evaluate the allelic variability of the bla locus in a representative collection of international epidemic MRSA clones and also, for comparative purposes, in a diverse collection of MSSA strains, in an attempt to establish evolutionary correlations between bla allotypes and β-lactam resistance phenotypes (i.e. between MRSA and MSSA), SCCmec types (i.e. polymorphisms in the mecA regulatory locus) and/or genetic lineages. MRSA lineages are much less diverse than MSSA lineages in terms of their genome content, a consequence of their more recent evolutionary history [19, 20] and, apparently, also due to some “”host barrier”" to the SCCmec acquisition [13]. These differences in genetic background variability were well illustrated in our collections since the international Diflunisal MRSA collection comprised eight lineages as defined by MLST clonal complexes, whereas
in the smaller and local MSSA collection 15 lineages were represented. In contrast to the genetic background GDC 0449 diversity, we could not detect significant differences between MSSA and MRSA in terms of the bla locus allelic variability. Actually, there were disparate subtle differences in terms of number of allotypes and number of point mutations per allotype: e.g. 11 vs 9 blaZ allotypes and 11.4 vs 14.7 SNP/allele in MRSA and MSSA, respectively. These subtle differences may reflect the more ancient evolutionary history of MSSA or a selective pressure to improve the bla locus activity in these strains. That is to say, although fewer bla types have been retained by the natural selection in MSSA, on average, these allotypes seem to have accumulated more adaptive mutations, in comparison to MRSA strains.