The band distribution of bacterial population in individual samples ranged from 20 to 26 (mean 22.40 ± 1.71 SD) in non-tumor where as 15 to 26 bands (mean 20.60 ± 3.10 SD) in tumor groups. The Mann–Whitney U test to compare the Shannon-Weaver indexes of diversity (H’) in non-tumor and tumor samples showed no significant differences (p > 0.05, two-tailed) in oral microbiota between two sample groups. The inter- group similarities were found to be 40% to 80% by cluster analysis (Figure 2). Most of the clinically distinct
samples (non-tumor and tumor) from the same patients clustered together with exception of one sample (184_N and 184_T) as seen in their intensity profiles. Figure 1 DGGE profile of microbial communities from two clinically distinct non-tumor and tumor
groups. N–Non-tumor; T–Tumor; Marker I & II: DGGE reference markers correspond to 16S rRNA gene fragments from quoted specific BYL719 manufacturer bacterial species [Marker I: 1. Fusobacterium nucleatum subsp. vincenti (ATCC 49256); 2. Fusobacterium nucleatum subsp. nucleatum (ATCC 25586); 3. Streptococcus sanguinis (ATCC 10556); 4. Streptococcus oralis (ATCC 35037); 5. Streptococcus salivarius (ATCC 7073); 6. Streptococcus mutans (UA 159); 7. Lactobacillus paracasei (ATCC 25598); 8. Porphyromonas gingivalis (ATCC 33277); 9. FDA approved Drug Library in vitro Actinomyces odontolyticus (ATCC 17929);10. Actinomyces Selleck MG132 naeslundii (ATCC 12104), Marker II: 1. F. nucleatum subsp. vincenti (ATCC 49256); 2. F. nucleatum subsp. nucleatum (ATCC 25586); 3. Bacteroides forsythus (ATCC 43037); 4. S. sanguinis (ATCC 10556); 5. S. oralis (ATCC 35037); 6. Veillonella parvula (ATCC 17745); 7. Prevotella intermedia (ATCC 25611); 8. Aggregatibacter actinomycemcomitans (ATCC 43717); 9. P. gingivalis (ATCC 33277); 10. A.odontolyticus (ATCC 17929); 11. A. naeslundii (ATCC 12104)]. Figure 2 Dendrogram representing the fingerprinting intensity profile
of two clinically distinct samples from non-tumor and tumor tissues. N–Non-tumor; T–Tumor. Similarity index (SI) was calculated based on the total number of high and low intensity bands per lane and position of band migration reflecting number of bands the two lanes have in common. The values signify similarities in bacterial composition between non-tumor and tumor groups (Table 1). The tumor samples (intra- group), 1457_T and 527_T showed total dissimilarity in their profiles despite sharing the same group. The band similarity correlation was highest in non-tumor and tumor tissue samples (inter- group), 142_N/142_T (77.27%) and 146_N/146_T (71.43%) from the same patient indicating that most of the microbiota were common at both the sites but there were changes in the bacterial composition. Chi-square test indicated significant differences in intra- and inter- groups bacterial profiles (X 2 = 10.76, p = 0.005).