St isolates was also performed using a multiplex PCR with C. jejuni NCTC C. coli and 11 168 St Strains controlled as RM 2228 on. A method according to Wang et al. was used, with minor modifications. The primers used were con UEs to identify genes C hipo jejuni, C. coli glyA, C. lari and Telaprevir VX-950 C. upsaliensis and C. fetus subsp sapB2. F Status. Primer sequences for MPCR are shown in Table 1. The PCR mixture for the reaction consisted of 12.5 l GoTaq master mix, 3 l 25 mM MgCl 2, 5.5 l of nuclease-free water, 2 l of template DNA and 2 l of primer mix. Denaturation at 95 for 30 seconds, annealing for 30 s to 59 and at 72 for 30 seconds, with a final Verl EXTENSIONS of the period w during 72: min was DNA amplification carried out with 30 cycles as follows 7 in a Peltier Thermal cycler.
15 aliquots

. The gel was visualized for 80 min run at 90 V and a UV transilluminator. The positions of the B Cantabria were determined visually and with respect to a molecular weight marker. 2.5. Production of Campylobacter-St Strains not identified sequences laced Campylobacter isolates that could not be identified by MPCR were prepared by sequential Age of 16S rRNA identified, using the method of Inglis and Cohen. The primers used were F and R TAC CTT GTT ACG Kingdom27 UNI1492 ACT 3 Each reaction mixture consisting of primer, GoTaq master mix, 25 mM MgCl 2, nuclease-free water and DNA template. The temperature cycle was an initial denaturation at 95 15 min, followed by 30 cycles at 94 for 30 s, 58 was followed for 1 min and ending 72 for 2 min, at 72 for 10 min.
The PCR products were purified using a QIAquick PCR kit according to manufacturer’s instructions. Purified PCR products were sent to Eurofins for sequential lacing. The sequence data were compared with the reference collections collection tool available to nucleotide BLASTN. DNA templates, as described above also be prepared for RAPD used using the method Ertas et al. The PCR was was prepared using the Peltier Thermal Cycler and the total volume of the reaction mixture consists of 25 l 2.5 l of template DNA, 12.5 l GoTaq master mix, 2.5 l 25 mM MgCl 2, OPA-11 and 0.5 l 7 l nuclease-free water. Denaturation at 94 rpm for 1 min, annealing at 37 for 1 min and extension at 72 for 1 min with a final maturity of the Verl EXTENSIONS 72 10: The amplification was obtained after 50 cycles with the following parameters.
15 aliquots of the PCR products were separated by agarose gel electrophoresis. The gel was run for 2 h at 90 V visualized and a UV transilluminator. Positions of the tape were visually analyzed with respect to a molecular weight marker. Band positions were defined presence of the DNA band and the absence of DNA bands. These values were in NTedit entered data to obtain a matrix and is then determined in the version 2.2 software NTSYSpc for the construction of dendrogram on the simple matching coefficient of the UPGMA cluster analysis to determine the relatedness of Campylobacter isolates. 2.7. Antimicrobial susceptibility of Campylobacter species, the disk diffusion method of Bauer et al. was used to the antibiotic resistance of 116 Campylobacter St mme against the following antimicrobial agents to determine ampicillin 10 g, 30 g of chloramphenicol, nalidix