1 H-Sn-cha Thurs Figure 2B, the molar ratio formed Ratio of 1:1 DOX PazPC trained to about 400 nm, the particle S in the range of the size E of the liposomes by a natural phosphatidylcholine. This suggests that RAAS System maintaining the complex of a molar K ratio of 1:1 nnte One Similar structure as the Anin Arzneimittelstabilit t micelle complex ion pair must be effectively formed between PazPC and drug form. If the conditions of complex formation are removed, the micelle is not stable and dissociates drug. This was the best information on the stability of t at pH 6.0, where only about 5% of DOX and IDA are associated with 14% of the micelle, compared to 62% of DOX and IDA 87% at pH 7 CONFIRMS, 0 PH-sensitive drug release delivery systems will be advantageous for targeting the tumor and the endosome escape from the space of solid tumors and intracellular Ren endosome compartments have a lower pH. Due to the st Amplifier hydrophobic IDA, IDA-loaded micelles PazPC an hour Here% EE, which then have included only a gr Ere number of molecules of IDA in the core of the micelle and the lower CMC am Lecular system. Accordingly, IDA loaded micelles PazPC a high relative stability of t against dilution. Our data show that when the cells were then treated with PazPC with free drugs pretreated, drug absorption was not obtained Ht. Moreover, none or only slightly improved cellular Re recording was carried out by simply mixing the drug and PazPC in the cell culture medium. Probably the improvement of the low absorption of the drug by simple mixing of drugs and PazPC induced as a consequence of the spontaneous formation of ion pairs and complex micelles between PazPC and drugs in cell culture medium because the pH is neutral. This phenomenon Ph Of a small improvement induced by a mixture of drugs and PazPC by the MTT assay was best CONFIRMS.
The IC50 of a simple mixture of free drug and PazPC on both sensitive and resistant cells P388/ADR P388 the same size Enordnung as that of free DOX or IDA. These observations suggest that in order to improve the cellular Re recording DOX or IDA, the drug in the micelles PazPC should be captured. Some oxidized phospholipids, such as PazPC eliminated almost two-thirds of oxidized phospholipids in oxidized LDL. Scavenger receptors on macrophages recognize OxPLs in oxLDL OxLDL uptake and mediation. W While the P388 mouse leukemia is Chemistry neoplastic cells of macrophage lineage, it is quite m Possible that the system based on micellar PazPC can his lead on the absorption and cytotoxicity t on these cell lines due to the scavenger receptor exert. Interestingly, the micellar drug formulations does not improve the absorption of drugs in normal cells from the bone marrow, suggesting specificity T of this system for the treatment of leukemia Chemistry. A recently published Ffentlichter report also showed selective enrichment of negatively charged liposomes mediated Wnt Pathway cytotoxic agents in leukemic Mix cells residing in the bone marrow via the scavenger receptor. It is important to have increased Hte expression of scavenger receptors in tumor cells from patients with leukemia Chemistry and leuk Mix cells have been reported. P388/ADR resistant cell is anf Llig for drug-loaded micelles PazPC received the P388 Ngliche cells both in terms of absorption and cytotoxicity t. These differences are between P388 and P388/ADR Probab.