pneumoniae strain

A1517 showing a unique capsular serotype [GenBank:BAF75773.1] [14]. The GT encoded by orf9 (KP03803) is predicted to be 298 aa long, with a best hit on NCBI BLASTP with a putative dTDP-rhamnosyltransferase from D. dadantii [GenBank:ADM97617.1] (63% identity, Table 1). {Selleck Anti-infection Compound Library|Selleck Antiinfection Compound Library|Selleck Anti-infection Compound Library|Selleck Antiinfection Compound Library|Selleckchem Anti-infection Compound Library|Selleckchem Antiinfection Compound Library|Selleckchem Anti-infection Compound Library|Selleckchem Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|buy Anti-infection Compound Library|Anti-infection Compound Library ic50|Anti-infection Compound Library price|Anti-infection Compound Library cost|Anti-infection Compound Library solubility dmso|Anti-infection Compound Library purchase|Anti-infection Compound Library manufacturer|Anti-infection Compound Library research buy|Anti-infection Compound Library order|Anti-infection Compound Library mouse|Anti-infection Compound Library chemical structure|Anti-infection Compound Library mw|Anti-infection Compound Library molecular weight|Anti-infection Compound Library datasheet|Anti-infection Compound Library supplier|Anti-infection Compound Library in vitro|Anti-infection Compound Library cell line|Anti-infection Compound Library concentration|Anti-infection Compound Library nmr|Anti-infection Compound Library in vivo|Anti-infection Compound Library clinical trial|Anti-infection Compound Library cell assay|Anti-infection Compound Library screening|Anti-infection Compound Library high throughput|buy Antiinfection Compound Library|Antiinfection Compound Library ic50|Antiinfection Compound Library price|Antiinfection Compound Library cost|Antiinfection Compound Library solubility dmso|Antiinfection Compound Library purchase|Antiinfection Compound Library manufacturer|Antiinfection Compound Library research buy|Antiinfection Compound Library order|Antiinfection Compound Library chemical structure|Antiinfection Compound Library datasheet|Antiinfection Compound Library supplier|Antiinfection Compound Library in vitro|Antiinfection Compound Library cell line|Antiinfection Compound Library concentration|Antiinfection Compound Library clinical trial|Antiinfection Compound Library cell assay|Antiinfection Compound Library screening|Antiinfection Compound Library high throughput|Anti-infection Compound high throughput screening| D. dadantii is a distantly related plant pathogen of the Enterobacteriaceae family. Interestingly, there is little similarity between orf9 and other K. pneumoniae sequences. The highest identity match (31%) is with a putative rhamnosyltransferase from strain VGH484 [GenBank:BAI43783.1]. The presence of the rmlBADC genes (previously discussed) together with the possible click here rhamnosyltransferases provides appealing evidence that L-rhamnose makes part of Kp13’s capsular structure. orf10, the third gene encoding a putative GT located in region 2 of the Kp13 cps cluster, is predicted to code for a 253 aa long protein with a conserved domain selleck chemicals of unknown function spanning amino acids 36 to 193 (Pfam accession no. PF04765). As with orf9, the best hit (57% identity, Table 1)

is also with a sequence encoding a putative GT from D. dadantii [GenBank:ADM97619.1]. There was no similarity between the orf10 (KP03802) product and other published Klebsiella sequences. Finally, the last GT from cps Kp13, termed orf19, is located on the 3’ end of the cps cluster and encodes a predicted 330 aa product. This protein has similarity with several uncharacterized GTs family 2 from different Enterobacteriaceae, including E. coli TA271 this website [GenBank:EGI36158.1] (58% identity), D. dadantii [GenBank:ADM97622.1] (38%) and Cronobacter sakazakii [GenBank:ABX51890.1] (34%). Only a general domain of the GTs family 2 was found in this protein, spanning amino acids 7 to 145 (Pfam accession no. PF00535). In silico serotyping Using molecular serotyping for the cps cluster, Brisse et al. [29] showed that very distinct PCR-RFLP patterns (C patterns) were obtained for most of the K serotypes, indicating that differences in antigenic specificity among serotypes are due to differences in cps gene content. Thus, we have also applied in silico molecular serotyping to determine the capsular serotype

of isolate Kp13. For this approach, the sequence between the primers published by Brisse et al. [29] was used to search in silico for restriction sites of the HincII endonuclease. This sequence spanned 12,031 bp from wzi to gnd, and the in silico restriction analysis identified 12 restriction sites, corresponding to 11 restriction fragments (Table 2). The fragments, ranging in size from 368 to 1,777 bp, were selected for analysis as suggested by Brisse et al. [29] (Table 2). The cps Kp13 RFLP pattern was compared to 102 previously published C patterns [29]. None of the reference patterns matched the one displayed by Kp13 (see Additional file 1). The similarity score for Kp13 was greater than 10.4 (MST cutoff value score ≥ 0.