T is an acceleration of 120 kV. The holder P-glycoprotein of an Oxford CT3500 cryo-probe was used to obtain the ceramic samples upright 75th Other samples were examined cryoholder in a Tecnai 12 G2 TEM with a Gatan 626th The samples were examined in an imaging mode at low doses to minimize the exposure to electron beam irradiation and damage. The images were digitally in the CM120 on a cooled CCD camera Gatan 791 multiscan, or in a Tecnai high res Solution UltraScan 1000 2k 2k cooled CCD camera included with the Digital Micrograph 3.6 Software. 2.7. Wide-angle R Ntgenbeugung XRD experiments with protein samples lyophilized protein / drug samples or crystalline celecoxib were measured using a Pulverr Ntgendiffraktometers Philips PW 3020 equipped with a graphite monochromator crystal. The operating conditions were CuK radiation, 40 kV, 40 mA in the range of 2 to 3 recording to 65, at room temperature. 2.8. Loading efficiency celecoxib isocratic HPLC analysiswas carried out at room temperature, using a LiChroCART 100 RP 18-S Column flow of 0.5 ml / min and UV detection at 254 nm.
The mobile phase consisted Secretase Signaling of acetonitrile: methanol: water 45:45:10 acetic acid water containing 0.5% and adjusted to pH 3.5 with NaOH. The mobile phase was t Made possible through a filter of 0.45 m. Celecoxib standards were prepared as follows: celecoxib in methanol was diluted with mobile phase to different final concentrations to achieve by injection of 50 L in the HPLC-S molecules. The standard calibration curve was constructed in the factory Ant Peakfl Surface of celecoxib versus concentration of celecoxib. For the determination of total drug spending, Proteinf Filling and Drug extraction procedure was performed. 140 l cold acetonitrile was added to 120 l casein micelle drug sample, vortexed for 1 min, and immersed for 20 min in zersto Enem ice. For the determination of non-encapsulated drug was a strong anionic exchange resin used to the encapsulated and the encapsulated drug to separate. To 50 mg of Dowex was added 100 l casein micelle drug sample were added and the mixture was vortexed for 1 minute.
Both extracts and the samples were treated Dowex 10 min at 3000 g centrifuged at 4. The whichever type Walls were collected and diluted with mobile phase, and 50 L was inserted into the HPLC-S Molecules injected. Concentrations, celecoxib, and non-encapsulated drug samples in total were extrapolated from the results of the standard curve. Encapsulated celecoxib concentration was calculated by subtracting the non-encapsulated drug concentration of total drug concentration. 2.9. Water content, water content was determined using a Metrohm Karl Fischer coulometer 831st The measurements were performed with celecoxib powder, casein powder, freeze-dried powder and empty and made drug-loaded nanocarriers. Third Results and discussion celecoxib is characterized by low discharge a water-hydrophobic drug Solubility. Its polar and nonpolar surface Chen Are 86.4 397.7 2 and 2. The drug is sold under the brand name Celebrex Cidofovir Experiments show that celecoxib a highly permeable drug that can be absorbed in the gastrointestinal tract, however, its resolution and high limiting a rate factor for the absorption of solid dosage forms are present. Thus, the drug kept in an amorphous state, such as f in our casein.