Overall fewer active areas were present when compared with the lowlanders. The horizontal section revealed active areas similar to those in the … The total activated areas in both lowlanders (Fig. ​(Fig.4A)4A) and highlanders (Fig. ​(Fig.4B)4B) were computed and expressed as voxels for comparison. The lowlanders showed an approximate 1.3× increase in voxels (Fig. ​(Fig.5)5) while working on this simple mental task when compared to the highlanders, and the lateral views on the brain MG132 templates of the two groups revealed larger activated areas in lowlanders than highlanders. A comparison of some of the active areas was shown in Figure ​Figure6A6A Inhibitors,research,lifescience,medical and B. The red and yellow areas indicated overlapping

active areas shared by both lowlanders and highlanders. Inhibitors,research,lifescience,medical The green and blue areas were recorded in lowlanders only with P < 0.001. Greater areas in both deep frontal and parietal lobes were activated in lowlanders than highlanders

(Fig. ​(Fig.6A).6A). Figure ​Figure6B6B revealed that while the right hemisphere was primarily involved in performing the mental task. More active cortical regions were found in the lowlanders (blue and green areas) than the activated areas shared by both high and lowlanders (red and yellow Inhibitors,research,lifescience,medical areas). Figure 4 Lateral computer brain templates of overall active brain regions in (A) lowlanders and (B) highlanders. Larger and more intense areas were observed Inhibitors,research,lifescience,medical in the lowlanders,

indicated by yellow over red colors (P < 0.001). Figure 5 Comparison of total voxels in the brains of highlanders versus lowlanders upon mathematical calculation (t-test, P = 0.003). Bars shown are mean ± SD. Figure 6 Computerized comparison of overall active brain regions between lowlanders and highlanders in (A) lateral and (B) horizontal views. Red and yellow areas present significant overlapping activated regions in both lowlanders and highlanders. Green and blue ... Discussion Our results indicate that the parietal area is one of the major areas involved in mathematical computation as documented by others Inhibitors,research,lifescience,medical (Dehaene et al. 1999, 2003; Andres et al. 2012). In addition, the area in front of the executive motor strip, a part of the premotor area is also involved even in simple calculation in this study. It is likely that both the programing and association are necessary steps in performing the task. More importantly, the lowlanders and highlanders Rutecarpine displayed subtle differences in the areas involved, indicating perhaps diversified brain functioning after adaptation of the highlanders upon centuries of evolution. Most interesting is perhaps that the highlanders could perform the same function of computing with fewer brain regions involved. This may be similar but not equal to athletes who were trained in high altitudes when returning to low levels exhibited better performance (Bailey and Davies 1997).