In Molecular Genetics of the Bacteria-Plant Interactions. Edited by: Pühler A. Berlin: Springer-Verlag; 1983:98–106.CrossRef 43. Kovach ME, Elzer PH, Hill DS, Robertson GT, Farris MA, Roop RM, Peterson KM: Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes. Gene 1995, 166:175–176.PubMedCrossRef 44. Datsenko KA, Wanner BL: One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci U S A 2000, 97:6640–6645.PubMedCrossRef 45. Ruiz-Argüeso T, Hanus FJ, Evans HJ: Hydrogen production and uptake by pea

nodules as affected by strains of Rhizobium leguminosarum. Arch Microbiol 1978, 116:113–118.CrossRef TGF-beta assay 46. Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, Goeke NM, Olson BJ, Klenk DC: Measurement of protein using bicinchoninic acid. Anal Biochem 1985, 150:76–85.PubMedCrossRef find more 47. NSC23766 price Sambrook J, Russell DW: Molecular Cloning: A Laboratory Manual. 3rd edition. N.Y.: Cold Spring Harbor; 2001. 48. Brito B, Palacios JM, Hidalgo E, Imperial J, Ruiz-Argüeso T: Nickel availability to pea (Pisum sativum L.) plants limits hydrogenase activity of Rhizobium leguminosarum bv. viciae bacteroids by affecting the processing of the hydrogenase structural subunits. J Bacteriol 1994, 176:5297–5303.PubMed Competing

interests The authors declare that they have no competing interests. Authors’ contributions MA carried out most of the experimental work and constructed the Tangeritin C-terminal deletion mutant. HM constructed most of the mutants and plasmids and performed initial analysis of protein-protein interactions. AB conceived the experiments on HupL stability. BB performed experiments with HupF mutant proteins. JI and TRA participated in the design of the study and in the final writing of the manuscript. JP coordinated the study and drafted the manuscript. All authors read and approved the manuscript.”
“Background Fungi are among the most diverse eukaryotic organisms on Earth, with nearly 10,000 named fungal species and an

estimated 1.5 to 5 million species that are yet to be defined [1, 2]. Fungi are also recognized as an important element in human microbiome research, clinical medicine, and as emerging pathogens [3–8]. However, methodological challenges have limited scientists’ and clinicians’ ability to detect and measure fungal abundance. Currently, fungal detection is performed through culturing [9], serological detection of antigens, such galactomannan in invasive aspergillosis [10, 11], and molecular test panels [12]. Yet, these methods lack broad-coverage and are not quantitative [4, 13]. Next-generation sequencing is an effective approach for detecting and characterizing fungi, but it is expensive, requires complex analyses, and is not quantitative [14, 15].