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“Background : Continuous monitoring systems have allowed determination of the time-to-positivity (TTP). We evaluated the clinical relevance of UP in the BACTEC9240 system (Becton-Dickinson, USA).\n\nMethods : A total of 2,354 vials of positive blood cultures were evaluated over 2 months. UP was monitored from each of BACTEC Plus Aerobic/F (BD) or Pediatric Plus/F and Lytic Anaerobic/F bottles, and the differential time-to-positivity (DTP) for blood samples drawn simultaneously via catheter

and a peripheral site was determined.\n\nResults : The average UP of the positive vials was 17.4 hr, and 79.9% and 95.2% of the vials showed positivity within 24 and 48 hr, respectively. While the average UP values for Aeromonas hydrophila, Bacillus cereus, Acinetobacter baumannii, and Streptococcus pneumoniae were less than 10 hr, those for Candida spp., anaerobes, Propionibacterium Protein Tyrosine Kinase inhibitor acnes, Corynebacterium spp, Bacillus spp. other than cereus, and coagulase-negative staphylococci were 35.3, 27.0, 56.8, 45.8, 23.0, and 26.3 hr, respectively. The negative predictive values of UP over 24 hr to predict Staphylococcus aureus among staphylococci and S. pneumoniae among a-hemolytic streptococci

were 76.7% and 100%, respectively. Enterobacteriaceae and Enterococcus faecalis showed shorter UP in anaerobic vials than in aerobic vials. DTP of more than 2 hr was observed for 27.8%, 72.2%, and 45.5% of S. aureus, S. epidermidis, and Candida spp.\n\nConclusions : UP can be used to discriminate pathogens and contaminants. The shorter UP in anaerobic vials of certain Enterobacteriaceae and Enterococcus sop. would facilitate further identification. DTP is useful for diagnosing catheter-related bloodstream infection

by S. aureus, S. epidermidis, and Candida spp. (Korean J Lab Med 2010;30:276-83)”
“Several virulence factors of Helicobacter pylori play crucial role in the pathogenesis of the infections. H.pylori iceA gene which is induced by the contact with epithelium during the attachment of bacterium to the gastric mucosa, possess two variants (iceA1 and iceA2). Although there are some data indicating the relationship Quisinostat solubility dmso between H.pylori iceA1 and peptic ulcer, this concept is still controversial. The aims of this study were to investigate the presence and prevalence of H.pylori iceA1 and iceA2 gene regions in the tissue samples of patients diagnosed as chronic gastritis and gastric cancer, and to evaluate whether any correlation existed between these genotypes and clinical manifestations. A total of 109 tissue samples obtained from chronic gastritis (n = 55) and gastric cancer (n = 54) patients whose H.pylori infections have been confirmed by histopathologic examination of biopsy samples, were included in the study. The presence of H.