CONCLUSION: We achieved intraoperative reduction of occipitocervical instability through a purely posterior approach. This technique adds a tool to the armamentarium of techniques used for the treatment of occipitocervical instability.”
“This study was designed to characterize the differential protein expression in the progeny of human liver cells surviving exposure to ionizing radiation. The progeny of irradiated cells were derived from a human liver cell line exposed to 0, 2, 4, or 6 Gy of 60Co -irradiation. Total protein of the cells was extracted by two-dimensional electrophoresis
(2-DE) and analyzed with ImageMaster 2D Platinum software. In total, 42 differentially expressed proteins from the progeny of irradiated cells were screened, of which 17 were identified by matrix assistant laser desorption ion-top flight-mass spectrometry (MALDI-TOF-MS) analysis. There were find more 4 upregulated and 13 downregulated proteins detected. The upregulated expression AZD1152 nmr of two proteins, mitochondrial heat-shock 60-kD protein (HSP60) and globin transcription factor 1 (GATA-1), was further confirmed by immunoblotting. Database
search revealed that these differentially expressed proteins may function in cell cycle regulation, cytoskeleton maintenance, stress response, and tumor metastasis, indicating an effect of radiation-induced genomic instability (RIGI) in the progeny of irradiated cells. Analysis on functional roles of the screened proteins may provide insight into further mechanistic investigations underlying molecular events induced by RIGI.”
“OBJECTIVE: The authors have developed a novel intraoperative neuronavigation with 3-dimensional (3D) virtual images, a 3D virtual navigation system, for neuroendoscopic surgery. The present study describes this technique and clinical experience with the system.
METHODS: Preoperative
imaging data sets were transferred to a personal computer to construct virtual endoscopic views with image segmentation software. An electromagnetic tracker was used to acquire the position and orientation of the tip of the neuroendoscope. Virtual endoscopic images were interlinked Urocanase to an electromagnetic tracking system and demonstrated on the navigation display in real time. Accuracy and efficacy of the 3D virtual navigation system were evaluated in a phantom test and on 5 consecutive patients undergoing neuroendoscopic surgery.
RESULTS: Virtual navigation views were consistent with actual endoscopic views and trajectory in both phantom testing and clinical neuroendoscopic surgery. Anatomic structures that can affect surgical approaches were adequately predicted with the virtual navigation system. The virtual semitransparent view contributed to a clear understanding of spatial relationships between surgical targets and surrounding structures. Surgical procedures in all patients were performed while confirming with virtual navigation.