N He TACC2 of the gene. The ARBS TACC2 has been found that are in a transcriptionally active chromatin region. The test BX-795 showed ChIP activated histone modifications of H3K4 mono-methylation and H3/H4 acetylation and recruitment of ligand-dependent Ngigen RNA polymerase II or phosphorylated Pol II To determine whether the ligand-dependent Independent Transkriptionsaktivit t ARBS TACC2 directly by AR is regulated, we examined the function of the androgen response elements in TACC2 ARBS involved. If two canonical sequences were identified by the ARBS TACC2 TRANSFAC. We constructed vectors, the luciferase JAK Signaling Pathway mutations of these sequences are compared and Transkriptionsaktivit Th with the luciferase vector TACC2 ARBS intact. Significant suppression of luciferase activation was observed in each of the mutant constructs. Androgen reactive ability TACC2 expression was examined in stimulated LNCaP cells by R1881 and dihydrotestosterone. TACC2 mRNA was detected in a Transient Induced ngigen way, and at approx Hr 2-fold 24 h was detected after R1881 treatment. Regarding the size E of the TACC2 isoforms was expressed mRNA in LNCaP cells According mainly derived from the short isoform, as defined by the design of PCR primers. Additionally Tzlich we investigated the reactivity Ability of androgen TACC2 protein expression in LNCaP cells using specimens of specific antibody That TACC2 two isoforms. Regulation of androgen-dependent Ngigen to TACC2 protein was after stimulation of LNCaP cells which Molekülgr E to the short isoform transiently expressed in 293T cells corresponds shown. DHT was also found to a rise in TACC2mRNAlevel more than 2.5 times 24 hours after treatment, and the induction was inhibited by causing the antiandrogen bicalutamide.
To further investigate the direct contribution of AR to TACC2 expression, we performed AR knockdown by small interfering RNA transfection. If the AR siRNA in a concentration sufficient to t Th AR protein was transfected, the mRNA level TACC2 by approximately 40% reduced. We also found that a remarkable Ausma Recruitment of AR to ARBS TACC2 in the VCAP cells is detected, another cell line AR positive prostate cancer and metastatic prostate cancer tissue at an advanced stage in the most recently available Published data ChIP Seq. We have also validated TACC2 induction in cells VCAP. Taken together, these results indicate that AR regulates gene expression, in a manner TACC2 ligand-dependent Independent prostate cancer. TACC2 f Promotes cell cycle progression in prostate androgen-dependent Ngigen proliferation of cancer to investigate the function of the function TACC2 prostate cancer, we altretamine reduced the endogenous expression of siRNA treatment TACC2. We used two siRNA sequences targeting different regions TACC2 and best Firmed that each siRNA transfection effectively suppresses the mRNA level TACC2 and the level of the protein in LNCaP cells TACC2. TACC2 knockdown was found that the increased proliferation of LNCaP cells activated in both basal and hormone levels to be reduced. In addition to determining the function of TACC2 cell cycle regulation, we performed cell cycle analysis by fluorescence-activated cell sorting. After 96 h siRNA treatment resulted in a FACS analysis that TACC2 silence to G2 / M leads