CD was rbent developed to separate and purify EGCG from green tea extracts. The adsorbents were prepared by elemental analysis and thermogravimetric analysis. The characterization results show that, under optimum conditions of preparation, the amount of silica GOPS GOPS modification about 0.10 0.18 mmol / g adsorbent GOPS and the amount of silica-CD BMS-582664 Brivanib alaninate was grafted on CD, was about 0, 02 CD 0.05 mmol / g adsorbent, so that about a CD molecule was fixed at four GOPs related molecules of silica gel. Crude extracts of green tea have concentrated by liquid-liquid extraction under the following conditions: Ethyl acetate as the extraction solvent L, water / l 1/1, the extraction temperature of 30 time, and the extraction of 2 h The purity of EGCG in green tea concentrated extracts from 6.0 to 36.0 8.
0% 40.0% with a high recovery rate of 80%. The concentrated extracts of green tea were then used as samples in the batch adsorption experiments and Chromatographies Column used. In batch BIX 02189 adsorption experiments with CD-bonded silica Acid adsorbent, the adsorption ratio Ratio of EGCG and adsorption was found to be markedly Ago as tea catechins and caffeine others. The high Adsorptionskapazit And selectivity T for EGCG in the tea catechins and caffeine are others on the specific interactions between the CD and EGCG, including the inclusion complexation, hydrogen bonding and interaction attributed to the interaction. The superior performance of the adsorption of tea catechins, particularly EGCG, on the successful transplantation of CD GOP silica particles can be a reliably Be ssiges criterion to characterize the CD silica product adsorbent.
In the experiments, the S Column chromatography, pr Preparative separation and purification performance of EGCG was evaluated. 22.5 g of the adsorbent was silica product CD in 220 mm L 15 mm diameter glass × Column. The column appears in the UI have polar organic use of methanol / acetonitrile / acetic Controlled acid as mobile phase. Were rst Excellent separation of EGCG and EC found with acetonitrile / methanol / acetic Acid 90/10/1. Then the sample was dissolved in acetonitrile St and on a pr Parative Ma Rod of about 0.8 mg / g of adsorbent. The optimum mobile phase is a three-phase gradient elution program consisting of acetonitrile / methanol / acetic Acid 90/10/1 at 0 20 min, 20 min in 100/0/1 67.5, and 67.
5 0/100 / 1 ml after min at a flow rate of 4 / min. The elution was found to ECG EGCG EGC C EC. The target compound was EGCG, thereby maintaining the successful species, with a purity of about 90% with a yield of about 90% under optimum conditions by chromatographic. The Productivity t of EGCG was about 6 mg per injection. The CD-bonded silica adsorbent can be scaled as a promising way to produce high-purity EGCG used with high recovery of green tea extract, epicatechin, epicatechin and. In addition, contains Lt GT small amounts of catechin and gallocatechin. Interestingly, the meta-analysis cited above reported that, unlike green tea, black tea consumption does not significantly associated with decreased risk of CHD. In the case of the effects of black tea, however, analysis showed an heterogeneity t in Europ European