As the scientific community continues to gain knowledge with respect to the genetic mechanisms involved in providing resistance to various antibiotics, the design of additional sets of degenerate primers will be possible and will provide further opportunities for the use of PCR to rapidly and efficiently detect antibiotic resistance genes in complex microbial environments, including the human gut microbiota. Availability of supporting data The data sets supporting results of this article are available in the LabArchives repository, [http://dx.doi.org/10.6070/Momelotinib clinical trial H42V2D1V].
Acknowledgements The authors wish to acknowledge ML323 the advice, assistance and protocols received from Dr. Brian Jones and Dr. Lesley Ogilvie regarding metagenomic sample preparation and analysis. Additionally the authors acknowledge the gift of control bacteria strains from the Smalla laboratory, JKI, Braunschweig. Fiona Fouhy is in receipt of an Irish Research Council EMBARK scholarship and is a Teagasc Walsh fellow. Research in the PDC laboratory is also supported by the Irish
Government under the National Development Plan through the Science Foundation Ireland Investigator award 11/PI/1137. References 1. Davies J, Davies D: Origins and evolution of Quisinostat mw antibiotic resistance. Microbiol Mol Biol Rev 2010, 74:417–433.PubMedCentralPubMedCrossRef 2. Abraham E, Chain E: An enzyme from bacteria able to destroy penicillin. Nature 1940, 146:837–837.CrossRef 3. Salyers AA, Gupta A, Wang Y: Human intestinal bacteria as reservoirs for antibiotic resistance genes. Trends Microbiol 2004, 12:412–416.PubMedCrossRef 4. Broaders E, Gahan CG, Marchesi JR: Mobile genetic elements of the human gastrointestinal tract: potential for spread of antibiotic resistance genes. Gut microbes 2013, 4:271–280.PubMedCrossRef 5. Dethlefsen L, Huse S, Sogin ML, Relman DA: The pervasive effects of an antibiotic on the human gut microbiota, as revealed by deep 16S rRNA sequencing. PLoS Biol 2008,
6:e280. 210.137/journal.pbio.0060280PubMedCentralPubMedCrossRef Erastin in vivo 6. Cotter P, Stanton C, Ross R, Hill C: The impact of antibiotics on the gut microbiota as revealed by high throughput DNA sequencing. Discov Med 2012, 13:193–199.PubMed 7. Sommer MOA, Dantas G, Church GM: Functional characterization of the antibiotic resistance reservoir in the human microflora. Sci 2009, 325:1128–1131.CrossRef 8. Mingeot-Leclercq MP, Glupczynski Y, Tulkens PM: Aminoglycosides: activity and resistance. Antimicrob Agents Chemother 1999, 43:727–737.PubMedCentralPubMed 9. Page MGP: Beta-Lactam Antibiotics. Antibiot Discov Dev 2012, 1:79–117.CrossRef 10. Tipper DJ, Strominger JL: Mechanism of action of penicillins: a proposal based on their structural similarity to acyl-D-alanyl-D-alanine.