The number of SGC7901/shRNA2

cells (25.60 ± 3.28, p < 0.01) passing through the Matrigel was markedly lower than the numbers of SGC7901 (55.80 ± 5.03) and SGC7901/shRNA-control (54.40 ± 4.35) cells. There was no significant difference between SGC7901/shRNA-control and SGC7901 (p > 0.05). Figure 4 Effects of CD147 specific shRNA on invasion of SGC7901 cells. (A)Crystal violet staining results of the lower surface filters showed that the cells passed through MLN8237 research buy the filter and attached to the lower side of the filter (400×). (B) The average number of cells that invaded through the filter was counted. The data were obstained from three independent experiments. *p < 0.01 compared with SGC7901 and SGC7901/shRNA-control. Silencing of CD147 in SGC7901 cells results in increased chemosensitivity to cisplatin CD147 was found to be overexpressed in multidrug resistance tumor cells and could confer resistance to some anti-tumor drugs. We

next investigated whether inhibition of CD147 by RNAi affected the sensitivity of SGC7901 cells to the anti-tumor drug cisplatin. As shown in Fig. 5, the inhibition rate in SGC7901/shRNA2 was markedly enhanced at all concentrations examined compared with SGC7901 and SGC7901/shRNA-control (p < 0.01). There was no significant difference between SGC7901/shRNA-control https://www.selleckchem.com/products/Adriamycin.html and SGC7901 (p > 0.05). Figure 5 Effects of CD147 specific shRNA on cisplatin sensitivity of SGC7901 cells. SGC7901, SGC7901/shRNA-control and SGC7901/shRNA2 were treated with various concentrations of cisplatin. Cell vialility was determined by MTT assay. *p < 0.01 compared with SGC7901 and SGC7901/shRNA-control. Discussion CD147, also designated EMMPRIN (extracellular matrix metalloproteinase inducer), is a cell surface glycoprotein which belongs to the immunoglobulin superfamily. As CD147 is highly expressed in most tumors and was shown to increase tumor invasion, most studies

so Selleck Rucaparib far focuses on its role in cancer progression. However, its expression is not limited to tumor cells and was shown to be expressed in many cell types, including haematopoietic, epithelial, endothelial cells and leukocytes [14]. Gene silencing by RNA interference has emerged as a powerful method that is useful for the study of functional genomics [15]. Here, we successfully transfected two shRNAs targeting CD147 gene into human gastric cancer cell line SGC7901. Two stable cell clones SGC7901/shRNA1 and SGC7901/shRNA2 were obtained. CD147 expression was effectively inhibited at both mRNA and protein levels by shRNA2, while the shRNA1 was less efficient. These results indicated that shRNA targeting different sites of the same mRNA might be different in silencing efficiency. We then examined the effect of CD147 silencing on the proliferation of SGC7901 cells. The proliferation potential of SGC7901/shRNA2 cells was suppressed compared with that of the control SGC7901 cells. Chen et al. and Jia et al.