Without a doubt the information presented in Table 1 exhibits the raise in the information of fatty acids in oil. The enhance in unsaturated 18,1, 18,2 and 18,3 fatty acids in both transgenic lines was detected. The highest enhance was measured in W92. forty transgenic line. Beside composition the larger accumulation of all fatty acids in transgenic seeds when in contrast towards the management was observed. The 31% and 40% increase of complete fatty acids content material in oil from transgenic plant W92. 40 and W92. 72, respectively, in contrast to the handle. Hence it’s recommended that accumulation of flavonoids in flax motive ably affect the fatty acids manufacturing in seeds. The next stage in oil examination was evaluation of its sta bility. Degree of lipid oxidation stability was determined by by measuring peroxide value and TBARS.

The TBARS measure ments exhibits lower by about 80% and 40% for oil from lines W92. forty and W92. 72, respectively, when selleckchem Pim inhibitor com pared to regulate. Also peroxide value is slightly reduce for transgenic plant. Since the flavonoid biosynthesis pathway is separated from fatty extra resources acids metabolism, probably the way in which by which the last is activated on CHS, CHI and DFR overex pression originates from nonspecific antioxidative perform of flavonoids. In other words accumulated antioxidative compounds in transgenic seeds partially extract with oil and therefore may well shield fatty acids towards oxidation. The query appeared no matter if the antioxidants accu mulate in oil from transgenic seeds. For that causes we measured the compound content that might defend fatty acids in oil against oxidation.

There was slight but not substantial big difference in antioxidants level in oil from transgenic seeds compared to regulate. Even so the stability MK-0752 of oil from transgenic plant is enhanced. So the last conclusion is the fact that fatty acids metabolic process modifications in transgenic seeds resulted from their safety throughout synthesis and seed maturation. This conclusion is surely an agreement describes it with the in vitro stu dies, the place unsaturated fatty acids were protected against oxidation when supplied with micromolar concentration of exogenously additional quercetin or b carotene. Biochemical analysis of seedcake extract Because the modifications in fatty acids composition and yield of oil had been detected it had been realistic to analyze the residual seed tissues just after oil pressing.
It was expected an accumulation of antioxidants from flavonoid pathway in seedcakes and as a result safety of fatty acids. The ana lysis was carried out on comparable methanol extract as it was carried out for seeds. The information presented in Figure 3 displays the sizeable increase of flavanones and flavonols likewise as anthocyanins level. Much like seed extract, the flavanone material in seedcake extract from W92. 40 and W92. 72 plant was improved by 53% and 55%, respec tively.