“BACKGROUND: Olive mill wastewaters (OMWs) are an important residue and several methods have been proposed for their treatment. RESULTS: Remarkable decolorization (similar to 63%) and phenol removal (similar to 34% w/w) from OMW was achieved. In glucose-based
flask sterile cultures, enrichment with OMWs increased ethanol and biomass production compared with cultures without OMWs added. Flask sterile and un-sterilized cultures demonstrated similar kinetic results. Batch-bioreactor trials performed showed higher ethanol and lower biomass quantities compared with the respective shake-flask experiments, while cultures used under un-sterilized conditions revealed equivalent results to the sterile ones. In non-sterile bioreactor cultures, OMWs addition enhanced biomass production
in comparison Selinexor chemical structure with culture with no OMWs added, whereas ethanol biosynthesis was not affected. The maximum ethanol quantity achieved was 52 g L1 (conversion yield per sugar consumed of 0.46 g g1) in a batch bioreactor non-sterilized trial with OMWglucose enriched medium used as substrate, that presented initial reducing sugars concentration at similar to 115 g L1. Fatty acid analysis of cellular lipids demonstrated that in OMW-based media, cellular lipids containing increased concentrations of oleic and linoleic acid were produced in comparison with cultures with no OMWs added. CONCLUSIONS: S. cerevisiae simultaneously produced bio-ethanol and biomass and detoxified OMWs, under non-sterile conditions. (c) 2012 Society of Chemical Industry”
“The accumulation of reactive oxygen species (ROS) is involved in plant cell learn more development, In plant, class III peroxidases are heme-containing enzymes encoded by a large multi-gene family participated in the release or consumption of ROS. The specific function of each member of the family is still elusive. Here, we showed that ROS was significantly generated during cotton fiber initiation and elongation, whereas, application of NADPH oxidase inhibitor diphenyleneiodonium (DPI) and peroxidase inhibitor salicylhydroxamic
acid (SHAM) to the wild-type cotton ovule culture significantly suppressed fiber growth, respectively. Their inhibitory VX-770 concentration effects were caused by the reduction of superoxide radical (O(2)(-)). Ten GhPOX genes (cDNAs) encoding cotton class III peroxidases were isolated, among them eight GhPOX genes were reported for the first time. Microarray analyses indicated that GhPOX1 was the mostly predominantly expressed in fast-elongating cotton fiber cells. Real-time quantitative PCR analysis revealed the transcript level of GhPOX1 was over 400-fold higher in growing fiber cells than in ovules, flowers, roots, stems and leaves. To reveal the role of GhPOX1 in plant development, its Arabidopsis orthologue arpox13 mutant was demonstrated to be defective in branch root development.