These absorbance ratios correspond roughly to the range of CR absorbance ratios (R) encountered in oceanic measurements. The absorbance

Alectinib ic50 measurements used to determine the ratios were well within the linear-response characteristics of the Cary 400 spectrophotometer. The temperature and salinity ranges were 278.13 ≤ T ≤ 308.27 K and 20 ≤ S ≤ 40. Initial estimates for the e1 term in Eq.  (2) were obtained by determining the e1 molar absorptivity ratio at a pH where the HI− form of the dye is dominant. Iterative calculations are necessary to account for absorbance contributions at 433 nm and 573 nm from the H2I and I2 − forms of the dye. The overlapping absorbance spectra of H2I, HI− and I2 − are shown in Fig. 1. A speciation model for T = 298.15 K and S = 35 was constructed using the K1 determined as described in Section 2.7 and the K2 reported by Byrne and Breland (1989). At a pH of 4.5, HI− is near

99.91% of the total CR concentration; the fractions of H2I and I2 − are 0.045% and 0.046%. Requisite e1 Pexidartinib molecular weight absorbance data (573A/433A) were determined with a 0.02 m acetate buffer solution at ionic strength of 0.7 m NaCl. No salinity dependence was observed for the very small e1 term. During preparation of the acetate/acetic acid buffer solution, pHf (free scale) was monitored with a ROSS combination electrode that had been calibrated on the free hydrogen ion scale by titrating a 0.7 m NaCl solution with standard HCl. Because the HI− absorbance signal includes contributions from the H2I and I2 − forms of the dye, the following equation was used to account for these contributions (see also derivation of Liu et al., 2011): equation(6) e1=εHI−573εHI−433=AHI−573/sHI−AHI−433/sHI−=AT573−AH2I573−AI2−573AT433−AH2I433−AI2−433where λεHI is the molar absorptivity at a given wavelength (λ) for the HI− form of the indicator, λAx is the absorbance at wavelength λ of total (T) indicator (all forms) or of individual indicator forms (H2I, HI−, or I2 −), s is the cell pathlength, and [HI−] is the concentration of the HI− form.

Expressing the absorbance terms in Eq. (6) in terms of molar absorptivities and total CR concentrations (IT) via K1 and K2, e1 can be written as follows: Janus kinase (JAK) equation(7) e1=AT573−εH2I573ITsH+2K1K21+H+K2+H+2K1K2−1−εI2−573ITs1+H+K2+H+2K1K2−1AT433−εH2I433ITsH+2K1K21+H+K2+H+2K1K2−1−εI2−433ITs1+H+K2+H+2K1K2−1 To obtain the K2 value required in this calculation, initial e1 estimates were used to obtain initial K2T estimates by solving Eq.  (2) for − log (K2Te2). The e2 term, required to calculate K2T from − log(K2Te2), was calculated as a function of temperature by using the HI− absorbance at λ = 433 nm in the solution used to determine e1 (i.e., acetate buffer of pH = 4.5 and 0.7 m ionic strength) and the absorbance at λ = 573 nm in the solution used to determine e3/e2 (i.e., modified synthetic seawater of pH = 12 and 0.7 m ionic strength).