Parameter estimation KU-60019 clinical trial reveals that condition (6) was fulfilled. Doing so, the course of PEP depends on the value of K20 (equilibrium constant of the reversible glycolytic reaction rgly). Figure 9 shows the course of PEP for different values of K20 (plot A) and the course of phosphorylated EIIA. As can be seen in Figure 9, an extremum is reached for a small value of K20 in the range of the growth rate considered here. In all other cases, PEP is monotonously increasing. The course of phosphorylated

EIIA shows a very low sensitivity with respect to K20 (plot B). The same is true for fructose-1,6-bisphosphate (data not shown). Figure Inhibitors,research,lifescience,medical 9 Left (plot A): course of PEP for different values of K20 (dashed curve: K20 = 0). Right (plot B): course of phosphorylated EIIA for different values of K20. K20 was varied between 0.05 and 0.5. Experimental data to verify the simulations are found. Several studies Inhibitors,research,lifescience,medical focus on single growth conditions or on specific stimulations of the system [18,19].

In [24] E. coli was starved Inhibitors,research,lifescience,medical for carbohydrates and nitrogen. This situation reflects a move from a high growth rate to a very low growth rate. Figure 4 in [24] shows time course data for PEP and fructose-1,6-bisphosphate for carbon starvation. After stimulation, PEP increases very fast up to a factor of 64 but then decreases, and after 8 hours the former steady state is almost reached; in contrast fructose-1,6-bisphosphate decreases fast and remains at the new steady state during the remaining time of the experiment. In [25] several stimulations were performed and PEP was measured. For a classical experiment when E. coli is growing on glucose and lactose, the dynamic of PEP could also be monitored. For Inhibitors,research,lifescience,medical both growth phases, the level of PEP is nearly constant. In an A-stat experiment different metabolites of central metabolism were monitored Inhibitors,research,lifescience,medical [15]. Although the data are noisy, the level of fructose-1,6-bisphosphate

and glyceraldehyde-3-phosphate show a monotone increasing correlation with the growth rate. To summarize, there is experimental evidence that the simulation results predicted using this model (see Figure 6) reflect the true intracellular behavior. With a newly designed strain that allows to adjust the level of PtsG, it old was possible to “move” from one branch of the characteristic curve of phosphorylated EIIA (non-PTS case) to the other branch (PTS case). The experiments are designed such that glucose is taken up by the PTS and also by non-specific uptake systems. With the data of these experiments, it was possible to approach different points on the uptake kinetics for parameter estimation. While the data for the degree of phosphorylation were taken from previous experiments, the data with the new strain confirmed the relationship already published.