, 2006) If venom is still measurable after antivenom has been ad

, 2006). If venom is still measurable after antivenom has been administered it is thought that this represents free venom and insufficient antivenom has been Talazoparib ic50 given. We have

previously made use of the same technique in vitro to show that the addition of increasing concentrations of antivenom to venom gives an exponential decrease in measurable free venom ( Isbister et al., 2007 and Isbister et al., 2011). The concentration of antivenom at which venom is no longer detectable can then be converted to a dose required for neutralisation. This approach appears to work well with Australian antivenoms where there is likely to be an excess of antivenom compared to venom, because the commercial antivenom is highly concentrated (O’Leary and Isbister, 2009) and the venom concentration Lumacaftor order in patients is low due to the small amount of venom delivered by elapids (Kulawickrama et al., 2010, Allen et al., 2012 and Isbister et al., 2012). Therefore, venom is rarely detectable after administration of even one vial of antivenom in Australian elapid envenoming

(Allen et al., 2012 and Isbister et al., 2012). In contrast to this, in many non-Australian snakes, and in particular vipers, the venom concentrations are much higher (10–100 fold) and they are not reduced to zero following the administration of antivenom in a proportion of cases (Phillips et al., 1988 and Ho et al., 1990). However, the persistence of venom, or in some cases recurrence of detectable venom, does not always appear to be associated with persistence of envenoming, such as coagulopathy. One explanation for this is that the venom being detected as “free”

venom is in fact bound venom or venom–antivenom (VAV) complexes where the ratio of antivenom to venom is low (1–1) so that the VAV complex can still bind to the enzyme immunoassay (EIA) microplate (Fig. 1A). The suggestion that the assay for free venom can also detect bound venom (or VAV) as well as free venom means that it is important to be able to detect bound venom or VAV complexes. Such Alanine-glyoxylate transaminase an assay would require an antibody to bind to the venom component of the VAV complex and an antibody to the antivenom (i.e. anti-horse antibodies for an equine antivenom). Fig. 1B shows such an assay where antibodies to the venom are attached to the microplate and conjugated anti-horse antibodies are used as the detecting antibody. The aim of this study was to develop an assay to measure the venom–antivenom (VAV) complex which will complement the free venom assay. We investigate the binding of venom and antivenom in vitro with the assay, which could potentially be used determine if antivenom has bound to venom in vivo.

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